N-(Hydroxyethyl)piperazine-N-2-hydroxypropanesulfonic acid

N-(Hydroxyethyl)piperazine-N-2-hydroxypropanesulfonic acid is a high-purity biological-buffer supplied for industrial and specialty chemical applications. Contact our team for specifications, packaging options and lead times.

1. Biological Buffering Agent

Part of the Good's buffer family, commonly used in biological and biochemical research to maintain stable pH levels, especially in slightly alkaline conditions.

Used in:

Cell culture media and biological assays

Protein purification and enzyme kinetics

Electrophoresis and molecular biology techniques

2. Pharmaceutical Formulations

Utilized to maintain pH in drug formulations, including injectables and topical solutions, where consistent buffering near neutral to slightly basic pH is required.

3. Diagnostic and Analytical Reagents

Incorporated in clinical diagnostic kits and biosensors to ensure reproducible reactions sensitive to pH fluctuations.

4. Biochemical and Molecular Biology Research

Supports enzymatic reactions and nucleic acid stability during in vitro experiments requiring stable alkaline pH.

1. Strong Buffering Capacity in Alkaline Range

Effective buffering between pH 7.6 and 9.0, which is ideal for many biochemical processes and enzyme activities that prefer slightly alkaline environments.

2. Low Metal Ion Interaction

Minimal chelation of essential divalent cations like Mg²⁺ and Ca²⁺, preserving metal ion availability for enzymatic cofactors.

3. Chemical and Thermal Stability

Stable across a wide temperature range and resistant to degradation, allowing for long-term storage and consistent experimental conditions.

4. Low UV Absorption

Transparent in UV-visible light spectrum, facilitating spectrophotometric assays without interference.

5. Biocompatibility

Non-toxic and non-interfering with cellular systems, suitable for live cell experiments and in vivo studies.

N-(Hydroxyethyl)piperazine-N′-2-hydroxypropanesulfonic acid (CAS 68399-78-0) is a reliable buffering agent offering stable and effective pH control in slightly alkaline ranges. Its excellent solubility, low interaction with metal ions, and compatibility with biological systems make it a valuable component in biochemical research, pharmaceutical formulations, and diagnostic applications. It supports consistent and reproducible results in diverse scientific and medical settings.

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