CAS · 75277-39-3
Product Overview
HEPES sodium salt is a high-purity biological-buffer supplied for industrial and specialty chemical applications. Contact our team for specifications, packaging options and lead times.
Product Specifications
| Appearance | White crystalline powder |
|---|---|
| Assay (T) | 98% ~ 101% |
| Solubility (0.1M aq.) | Clear, colorless solution |
| Loss on drying | 3.0% max |
| pH (1% aq.) | 9.5 ~ 10.5 |
| Storage | Room temperature |
Applications
HEPES sodium salt is a widely used zwitterionic buffering agent in biochemical, molecular biology, and pharmaceutical research. It maintains physiological pH in cell culture media, enzyme assays, protein purification, and molecular biology experiments. In cell biology, it stabilizes pH in culture conditions for mammalian cells, supporting optimal growth and metabolic activity. In enzymology and biochemical assays, HEPES sodium salt provides stable pH control for sensitive reactions, minimizing variability and preserving enzyme activity. It is also used in pharmaceutical formulation research, diagnostic reagent preparation, and buffer systems for chromatography and electrophoresis. Its compatibility with aqueous systems and biological molecules makes it an essential reagent in both research and industrial biotechnology.
Benefits
The benefits of HEPES sodium salt arise from its excellent buffering capacity, minimal interference with biological processes, and chemical stability. It maintains pH effectively in the physiological range (pH 6.8–8.2) even under temperature changes or metabolic activity, ensuring consistent experimental conditions. HEPES is highly water-soluble, non-toxic to cells at recommended concentrations, and does not chelate metal ions or react with biological molecules, reducing experimental artifacts. Its stability under storage and experimental conditions allows reproducible results in long-term studies. The sodium salt form enhances solubility and ease of preparation in aqueous solutions, supporting accurate dosing and buffer formulation.
Conclusion
HEPES sodium salt is a versatile and reliable buffering agent widely used in cell culture, enzymology, molecular biology, and pharmaceutical research. Its strong pH-stabilizing properties, biocompatibility, and chemical stability make it essential for maintaining consistent experimental conditions and reliable results. By supporting precise pH control, enzymatic activity, and biological compatibility, HEPES sodium salt continues to play a critical role in modern biochemical, molecular, and pharmaceutical applications.
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