CAS · 39946-25-3
Product Overview
MES potassium salt is a high-purity biological-buffer supplied for industrial and specialty chemical applications. Contact our team for specifications, packaging options and lead times.
Product Specifications
| Appearance | White Crystalline Powder |
|---|---|
| Assay (Titration) | 99.0% min |
| Water Content | 1% max |
| pH (1% Di H2O, 25℃) | 8.5-9.5 |
| Solubility (1M water) | Clear Colorless Solution |
| A280 (0.1M water) | 0.040 max |
| A260 (0.1M water) | 0.050 max |
| Heavy metals (Pb) | 5 ppm max |
Applications
1. Biological and Biochemical Buffer
MES potassium salt is widely used as a Good's buffer in biological research to maintain stable pH in the slightly acidic range.
Commonly used in:
Cell culture media
Enzyme assays and protein purification
Molecular biology protocols where precise pH control is essential.
2. Pharmaceutical and Diagnostic Formulations
Used to buffer pH in drug formulations and in vitro diagnostic reagents that require a consistent acidic pH.
3. Electrophoresis
Applied in polyacrylamide gel electrophoresis (PAGE) and other electrophoretic techniques optimized for slightly acidic conditions.
4. Biochemical Assays
Supports reactions and assays involving enzymes and biomolecules that are active or stable in the pH 5.5–6.7 range.
Benefits
1. Optimal Buffering at Slightly Acidic pH
Provides excellent pH stability near 6.1, which is ideal for many biological processes sensitive to minor pH changes.
2. Low Metal Ion Binding
Exhibits minimal interaction with divalent metal ions, preserving enzyme cofactors and metal-dependent activities.
3. High Solubility
Easily dissolved in aqueous solutions, facilitating convenient buffer preparation.
4. Chemical and Thermal Stability
Maintains buffering capacity under a variety of experimental conditions, ensuring reliable results.
5. Low UV Absorbance
Does not interfere with UV spectrophotometric measurements, allowing accurate nucleic acid and protein quantification.
Conclusion
MES potassium salt (CAS 39946-25-3) is a widely utilized biological buffer optimized for maintaining stable pH in the slightly acidic range (5.5–6.7). Its excellent buffering capacity, low metal ion affinity, and chemical stability make it an indispensable tool in biochemical assays, cell culture, pharmaceutical formulations, and electrophoresis, ensuring reproducibility and precision in a broad array of life science applications.
Previous
MES Sodium Salt丨CAS 71119-23-8
Next