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Product Line: Life Science

PIPES monosodium salt

CAS 10010-67-0

Molecular structure of PIPES monosodium salt

CAS · 10010-67-0

01

Product Overview

PIPES monosodium salt is a high-purity biological-buffer supplied for industrial and specialty chemical applications. Contact our team for specifications, packaging options and lead times.

02

Product Specifications

Appearance White crystalline powder
Assay 99–101%
Water 3% max
pH (1% aqueous) 4–5
Solubility (0.1M aqueous) Colorless and clear
UV Absorbance (0.1M aqueous) 260 nm: 0.05 max 280 nm: 0.04 max
   
03

Applications

1. Biological Buffering Agent

PIPES monosodium salt is a Good's buffer, used to maintain stable pH in the near-neutral range, especially in biological and biochemical environments.

Commonly used in:

Cell and tissue culture media

Enzyme assays and protein purification

Nucleic acid handling

Immunoassays and Western blotting

2. Electrophoresis Systems

Acts as a buffer in gel electrophoresis, particularly PAGE, due to its effective buffering and low interference with biological molecules.

3. Pharmaceutical and Diagnostic Applications

Applied in drug formulation buffers, diagnostic reagents, and biologic preservation, where pH stability and biological inertness are essential.

04

Benefits

✅ Stable pH Near Neutral

With a pKa near 6.76, it is ideal for experiments requiring tight pH control in physiological and biochemical conditions.

✅ Low Metal Ion Binding

Has low affinity for divalent metal ions like Mg²⁺ and Ca²⁺, making it safe for enzyme and cofactor systems.

✅ Water Solubility

Easily soluble in water, allowing convenient preparation of buffer solutions.

✅ Minimal UV Absorbance

Does not interfere with UV absorbance-based measurements, supporting clean detection of DNA, RNA, and proteins.

✅ Chemically Stable

Provides excellent thermal and chemical stability, enabling reproducible experimental conditions.

05

Conclusion

PIPES monosodium salt (CAS 10010-67-0) is a versatile and reliable biological buffer, optimized for pH control in the 6.1–7.5 range. Its solubility, low metal ion interaction, and stability make it essential in life science research, diagnostics, biotechnology, and pharmaceutical applications, where precise, reproducible buffering is required without interference in sensitive biological processes.

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PIPES Disodium Salt丨CAS 76836-02-7