5-Aminofluorescein

5-Aminofluorescein is a high-purity fluorescein supplied for industrial and specialty chemical applications. Contact our team for specifications, packaging options and lead times.

1. Fluorescent Labeling and Bioconjugation

The primary amine makes 5-aminofluorescein ideal for covalent attachment to biomolecules, such as proteins, peptides, or nucleic acids.

Reacts readily with carboxyl groups (via EDC/NHS chemistry) and activated esters, forming stable amide bonds.

Commonly used to prepare fluorescently labeled antibodies, enzymes, and probes.

2. Fluorescence Microscopy and Flow Cytometry

Used to create fluorescent bioconjugates for detection of specific targets in microscopy and flow cytometry.

Allows high-sensitivity detection in cell imaging, receptor binding studies, and protein tracking.

3. Fluorescent Sensors and Biosensors

Incorporated into molecular sensors to detect pH, metal ions, enzymatic activity, or biomolecular interactions.

The amine group can serve as a functional site for tethering to sensor platforms.

4. Chemical and Biochemical Assays

Used in enzyme assays, binding assays, and molecular diagnostics to track interactions via fluorescence changes.

Its fluorescence is stable across a range of assay conditions.

5. Surface and Polymer Functionalization

5-Aminofluorescein can be immobilized onto surfaces, polymers, nanoparticles, or hydrogels, creating fluorescent materials for bioimaging or detection.

1. Functional Reactivity

The primary amino group allows targeted conjugation while maintaining strong fluorescein fluorescence.

Enables versatile chemical modification for biological, analytical, and material applications.

2. High Fluorescence Intensity

Emits strong green fluorescence under blue-light excitation (peak emission ~515 nm), suitable for sensitive detection.

3. Water Solubility and Biocompatibility

Retains hydrophilic properties, making it compatible with biological systems.

Generally non-toxic at working concentrations.

4. Photostability and Sensitivity

Offers good photostability for microscopy and long-term fluorescence measurements.

Detectable at low concentrations, supporting trace-level analysis.

5-Aminofluorescein (CAS 3326-34-9) is a versatile fluorescent dye that combines the bright, stable fluorescence of fluorescein with the reactive functionality of a primary amine. It is widely used for bioconjugation, fluorescence imaging, biosensor development, and molecular diagnostics. Its reactivity and strong signal make it a valuable tool in both biological research and analytical chemistry.

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