CAS · 3326-32-7
Product Overview
Fluorescein-5-isothiocyanate is a high-purity fluorescein supplied for industrial and specialty chemical applications. Contact our team for specifications, packaging options and lead times.
Product Specifications
| Appearance: | Yellow Powder |
|---|---|
| Absorption Maximum: | 494±5nm |
| Extinction Coefficient (cm⁻¹M⁻¹): | 65000±5000 |
| Purity (HPLC): | 95% min (254nm) |
Applications
1. Fluorescent Labeling of Biomolecules
FITC is extensively used to label antibodies, proteins, peptides, and nucleic acids for detection in various immunoassays.
Enables visualization and tracking of biomolecules in flow cytometry, immunofluorescence microscopy, and fluorescence-activated cell sorting (FACS).
2. Immunohistochemistry and Immunocytochemistry
Facilitates specific detection of cellular components and biomarkers by conjugating to antibodies.
Used in clinical diagnostics and research to identify cellular antigens and tissue structures.
3. Fluorescence Microscopy
Labeled biomolecules emit bright green fluorescence, enabling high-resolution imaging of cells and tissues.
FITC conjugates are used in live-cell imaging and fixed sample analysis.
4. Flow Cytometry
Widely used fluorochrome for multicolor labeling of cells, allowing the simultaneous analysis of multiple cellular markers.
5. Fluorescence-Based Assays
Integral to ELISA, Western blotting, and other assays that rely on fluorescence detection for sensitive quantification.
Benefits
1. Strong and Bright Fluorescence
FITC exhibits high fluorescence quantum yield and strong green emission, increasing assay sensitivity.
2. Covalent Labeling
The isothiocyanate group forms stable thiourea bonds with primary amines in biomolecules, ensuring durable conjugates.
3. Versatility
Applicable to a wide range of biomolecules due to the universal presence of amine groups.
Compatible with many biological protocols and imaging platforms.
4. Established and Well-Characterized
FITC is a well-established fluorescent probe with extensive literature, protocols, and commercial availability.
5. Multiplexing Capability
Used in combination with other fluorophores for multicolor flow cytometry and microscopy.
Conclusion
Fluorescein-5-isothiocyanate (FITC, CAS 3326-32-7) is a highly effective fluorescent labeling reagent widely used in biological research and clinical diagnostics. Its ability to covalently bind proteins and other biomolecules via the reactive isothiocyanate group, combined with its bright green fluorescence, makes FITC invaluable for immunolabeling, fluorescence microscopy, flow cytometry, and sensitive bioassays.
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