CAS · 144089-97-4
Product Overview
2-Fluoro-2-deoxy Guanosine (n-ibu) CED Phosphoramidite is a high-purity nucleosides-nucleotides supplied for industrial and specialty chemical applications. Contact our team for specifications, packaging options and lead times.
Product Specifications
| Appearance: | Off-white or white powder |
|---|---|
| Purity (HPLC): | 98% min |
| TLC: | Two spot |
| Solution Clarity Test: | Clear |
| 31P NMR Purity: | 98% min |
| Water Content: | 0.5% max |
Applications
1. Solid-Phase Oligonucleotide Synthesis
Enables site-specific incorporation of 2′-F-dG into synthetic oligonucleotides.
Compatible with standard automated synthesis platforms for producing chemically stabilized RNA/DNA hybrids.
2. RNA Therapeutics Development
Widely used in:
siRNA (small interfering RNA)
Antisense oligonucleotides (ASOs)
RNA aptamers
Improves biological half-life and binding affinity in vivo, enhancing therapeutic potential.
3. Structural and Functional RNA Studies
Mimics natural RNA structure while allowing study of RNA–protein interactions, enzymatic activity, and molecular recognition under biologically relevant conditions.
4. Diagnostic Applications
Applied in the design of high-affinity nucleic acid probes for diagnostic assays including qPCR, FISH, and biosensors where enhanced hybrid stability is crucial.
Benefits
✔️ Increased Nuclease Resistance
The 2′-fluoro substitution blocks ribonuclease-mediated degradation, offering significant improvement in oligo stability, especially in serum or intracellular environments.
✔️ Enhanced Hybridization Properties
2′-F modifications increase thermal stability (Tm) and binding strength, improving specificity for target RNA or DNA sequences.
✔️ Lower Immunogenicity
Modified oligonucleotides exhibit reduced activation of innate immune sensors, such as toll-like receptors (TLRs), making them suitable for therapeutic use.
✔️ Efficient and High-Purity Synthesis
Protecting groups (N-iBu and CED) enable clean, high-yield synthesis with excellent coupling efficiency in automated synthesis protocols.
✔️ RNA-Like Behavior
Incorporation of 2′-F-dG into oligos creates RNA mimics with DNA backbone-like chemical stability, making them useful in RNA mimicry and hybrid systems.
Conclusion
2′-Fluoro-2′-deoxyguanosine (N-iBu) CED Phosphoramidite (CAS 144089-97-4) is a specialized building block used for synthesizing 2′-fluoro-modified oligonucleotides with enhanced biostability, affinity, and therapeutic value. It's a key tool in RNA interference, antisense therapy, and molecular diagnostics, offering resistance to nucleases, reduced immune stimulation, and high synthesis efficiency. Ideal for advancing oligonucleotide-based drug development and functional RNA research.
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