CAS · 29915-38-6
Product Overview
TAPS is a high-purity biological-buffer supplied for industrial and specialty chemical applications. Contact our team for specifications, packaging options and lead times.
Product Specifications
| Appearance | White to off white crystalline powder |
|---|---|
| Assay (T) | 99%–101% |
| Loss on drying | 1% max |
| Melting Point (MP) | 230–235℃ |
| Solubility (0.1M aqueous) | Clear, colorless solution |
| pH (1% aqueous solution) | 5–7 |
| Ultraviolet absorbance (260nm, 0.1M aqueous) | 0.05% max |
| Ultraviolet absorbance (280nm, 0.1M aqueous) | 0.03% max |
| Heavy metals (as Pb) | 0.0005% max |
Applications
1. Biological and Biochemical Buffer
TAPS is part of Good's buffers, a family of zwitterionic buffers ideal for biological research.
Commonly used to:
Stabilize pH in protein purification and enzyme activity assays
Maintain stable conditions in cell culture media
Buffer solutions for nucleic acid and molecular biology techniques
2. Electrophoresis
Frequently used in polyacrylamide gel electrophoresis (PAGE), especially when alkaline pH is needed for optimal separation of proteins or nucleic acids.
3. Pharmaceutical and Diagnostic Applications
Employed in formulation of biologics and diagnostic reagents to ensure pH-sensitive components remain active and stable.
4. Industrial Biotechnology
Used in biocatalysis and fermentation processes where buffering around pH 8–9 is crucial for enzyme function and metabolic activity.
Benefits
✅ Effective Buffering in Alkaline pH
With a pKa of ~8.4, TAPS is ideal for biological systems that function best at slightly basic pH, including many enzymes and cellular processes.
✅ Low Metal Ion Interaction
TAPS has minimal affinity for divalent metal ions (Mg²⁺, Ca²⁺), preserving their availability for enzymatic cofactors or signaling pathways.
✅ UV Transparency
Low absorbance at 260/280 nm makes TAPS compatible with spectrophotometric assays involving nucleic acids and proteins.
✅ High Aqueous Solubility
Readily dissolves in water, allowing easy preparation of buffer solutions with consistent and reproducible pH control.
✅ Non-toxic and Biocompatible
Suitable for live cell and tissue systems, offering low interference with biological assays.
Conclusion
TAPS (CAS 29915-38-6) is a reliable and biocompatible alkaline buffer widely used in biochemistry, molecular biology, electrophoresis, and pharmaceutical applications. Its excellent stability, minimal interference with biomolecules, and effective buffering range make it an essential tool for scientists requiring precise pH control in neutral to slightly alkaline environments.
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