CAS · 5625-37-6
Product Overview
PIPES丨5625-37-6 is a buffering agent used to maintain the pH of biological and biochemical systems. It is widely used in cell culture, biochemical assays, and molecular biology applications due to its stability and minimal reactivity.
Product Specifications
| Property | Specification |
|---|---|
| Appearance | White powder or crystalline powder |
| Assay | 99% min |
| Water | 0.5% max |
| Humidity (LOD) | 1.0% max |
| Solubility (0.5M in 1N NaOH) | Colorless and clear |
| pH (1.0M aqueous) | 6.6 - 7.0 |
| UVA/260nm | 0.15 max |
| UVA/280nm | 0.13 max |
Applications
1. Biochemical and Biological Research
● Cell Culture Medium: PIPES is used in cell culture media to maintain the pH in the physiological range, promoting optimal growth conditions.
● Enzyme Reactions: Commonly utilized in enzyme-based assays and biochemical reactions to stabilize pH during procedures that require precise pH conditions.
2. Molecular Biology and Biotechnology
● Electrophoresis: Used in gel electrophoresis and protein analysis as a buffering agent in running buffers for separating proteins, nucleic acids, and other biomolecules.
● PCR: Plays a role in polymerase chain reaction (PCR) and other amplification techniques to maintain the pH stability during amplification.
3. Industrial and Environmental Applications
● Buffer in Water Treatment: Can be used as a buffering agent in water treatment processes to maintain stable pH levels.
● Cosmetic Formulations: Employed in the cosmetics industry as a pH stabilizer in lotions and creams.
4. Pharmaceutical Formulations
● Injectable Solutions: Used in pharmaceutical formulations for injectable solutions to ensure stability at physiological pH.
● Pharmacological Research: Used in drug testing, where the pH stability is critical for maintaining the integrity of biologically active substances.
Benefits
1. pH Stability
● Provides excellent pH buffering capacity over a wide range, especially in biological conditions.
2. Minimal Reactivity
● Non-toxic and non-reactive with biological molecules, making it ideal for cell cultures, enzyme assays, and biological experiments.
3. Biocompatibility
● PIPES is biologically compatible, making it suitable for molecular biology and biotechnological applications where maintaining the biological environment is crucial.
4. Wide Application Range
● Due to its low interference with biochemical processes, it is applicable across many fields, including biotechnology, pharmaceuticals, and environmental science.
Conclusion
PIPES丨5625-37-6 is a highly effective buffering agent with a wide range of applications in biochemical research, molecular biology, and pharmaceutical formulations. Its ability to maintain pH stability and minimal reactivity makes it a valuable tool in laboratory studies and industrial processes where pH control is critical.
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