CAS · 251647-53-7
Product Overview
2′-O-MOE-N6-Benzoyl-Adenosine Phosphoramidite is a high-purity nucleosides-nucleotides supplied for industrial and specialty chemical applications. Contact our team for specifications, packaging options and lead times.
Product Specifications
| Appearance: | Off white to light yellow amorphous powder |
|---|---|
| Solubility Clarity: | 0.2M solution in acetonitrile is free from undissolved particulate |
| Identity (by 1H NMR): | To conform structure |
| Purity (HPLC): | 98% min |
| Purity (31P NMR): | 98% min |
| Residual Solvent: | 3% max |
| Moisture Content: | 0.5% max |
Applications
1. Synthesis of Modified Oligonucleotides
Enables the incorporation of 2′-O-MOE adenosine into RNA or antisense oligonucleotides (ASOs).
Used in solid-phase synthesis to build chemically stabilized oligos with enhanced therapeutic potential.
2. Antisense Therapeutics
2′-O-MOE modifications improve resistance to nucleases, increasing in vivo stability.
Enhance binding affinity to complementary RNA, improving potency.
Reduce off-target effects and toxicity by increasing specificity.
3. Research in Gene Regulation
Useful for creating modified oligos to study gene knockdown and splicing modulation.
Helps elucidate mechanisms of RNA interference and antisense-mediated gene silencing.
4. Diagnostic and Molecular Tools
Incorporated into probes for hybridization assays, PCR primers, and molecular diagnostics requiring high specificity and stability.
Benefits
✔️ Increased Nuclease Resistance
The 2′-O-MOE modification confers enhanced protection against enzymatic degradation, prolonging oligonucleotide half-life.
✔️ Improved Binding Affinity
MOE groups increase thermal stability (Tm) of duplexes, resulting in stronger and more specific hybridization.
✔️ Reduced Immunogenicity
2′-O-MOE modified oligonucleotides tend to elicit minimal immune responses, improving safety profiles.
✔️ High Synthesis Efficiency
N6-benzoyl and phosphoramidite protecting groups ensure efficient, high-yield coupling during synthesis.
✔️ Versatility
Compatible with a wide range of oligonucleotide chemistries and sequences, facilitating diverse research and therapeutic applications.
Conclusion
2′-O-MOE-N6-Benzoyl-Adenosine Phosphoramidite (CAS 251647-53-7) is a vital reagent for synthesizing 2′-O-methoxyethyl-modified oligonucleotides, particularly in the development of antisense therapeutics. It enhances nuclease resistance, binding affinity, and reduces immunogenicity, leading to more stable and effective RNA-targeting drugs. Its application spans molecular biology research, diagnostics, and pharmaceutical oligonucleotide synthesis, making it a key tool in advancing RNA-based technologies.
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