CAS · 3767-28-0
Product Overview
4-Nitrophenyl alpha-D-glucopyranoside is a high-purity pharmaceutical-intermediates supplied for industrial and specialty chemical applications. Contact our team for specifications, packaging options and lead times.
Product Specifications
| Appearance: | Light yellow powder |
|---|---|
| Purity (HPLC): | 98% min |
| 1H NMR (D2O): | Conforms to structure |
| Specific optical rotation [α]20D (C=1 in water): | +210 to +220° |
Applications
4-Nitrophenyl alpha-D-glucopyranoside is widely used as a chromogenic substrate for alpha-glucosidase and related enzyme assays. When hydrolyzed by the enzyme, the nitrophenyl group is released as 4-nitrophenol, which exhibits a yellow color that can be quantitatively measured using spectrophotometry. This property makes it valuable in biochemical research, drug discovery, and enzyme kinetics studies, where accurate monitoring of enzymatic activity is required. It is also used in screening assays for inhibitors of glycosidases, which are important targets in the treatment of diabetes, viral infections, and lysosomal storage disorders. Additionally, it finds application in microbiological assays to detect bacterial or fungal glycosidase activity in environmental or clinical samples.
Benefits
The benefits of 4-nitrophenyl alpha-D-glucopyranoside include its sensitivity, reproducibility, and ease of use in enzymatic assays. The chromogenic response allows rapid and precise quantification of enzyme activity, enabling high-throughput screening and kinetic analysis. Its high solubility in aqueous media ensures uniform reaction conditions, while the stability of the glycosidic bond prevents non-enzymatic hydrolysis during storage or handling. The compound's specificity for alpha-glucosidases provides reliable results in complex biological samples, and its compatibility with standard laboratory equipment makes it convenient for routine use.
Conclusion
In summary, 4-nitrophenyl alpha-D-glucopyranoside is a key chromogenic substrate for studying alpha-glucosidase and related enzymes. Its ability to produce a measurable color change upon enzymatic hydrolysis makes it an indispensable tool in biochemical research, drug discovery, and microbiological analysis. With its stability, solubility, and specificity, it provides reliable, sensitive, and convenient measurements of enzyme activity in a wide range of scientific applications.
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