CAS · 108321-27-3
Product Overview
PIPES dipotassium salt is a high-purity biological-buffer supplied for industrial and specialty chemical applications. Contact our team for specifications, packaging options and lead times.
Product Specifications
| Appearance | White crystalline powder |
|---|---|
| Assay | 99% - 101% |
| Water content | 3.0% max |
| Solubility (1.0M aqueous) | Colorless, transparent |
| Chloride (Cl) | 0.05% max |
| Sulfate (SO₄) | 0.005% max |
| Heavy metal (Pb) | 0.0005% max |
| Ultraviolet absorbance / 260nm | 0.05 max |
| Ultraviolet absorbance / 280nm | 0.04 max |
Applications
1. Biological and Biochemical Buffer
A popular Good's buffer, PIPES dipotassium salt is widely used for maintaining stable pH near neutrality in:
Cell culture media
Protein purification and enzyme reactions
DNA/RNA electrophoresis and molecular biology protocols
2. Electrophoresis Systems
Used in polyacrylamide gel electrophoresis (PAGE) and Western blotting to stabilize the pH during protein separation.
3. Pharmaceutical and Diagnostic Applications
Included in drug formulations, diagnostic test kits, and bioreagents that require consistent pH in the physiological range.
4. Industrial Biotechnology
Used in bioprocessing and fermentation systems that require tightly controlled, near-neutral pH conditions for enzyme or microbial stability.
Benefits
✅ Ideal pH Range for Biological Systems
With a pKa around 6.76, PIPES-K₂ provides precise buffering in the slightly acidic to neutral pH range, suitable for most biological experiments.
✅ Low Metal Ion Binding
Does not chelate metal ions significantly, preserving the availability of cofactors (e.g., Mg²⁺, Ca²⁺) essential for enzyme activity.
✅ High Water Solubility
Easily dissolves in aqueous media, making it convenient for buffer preparation.
✅ Chemical and Thermal Stability
Stable across a range of temperatures and chemical environments, ensuring reproducible results in diverse conditions.
✅ Low UV Absorbance
Minimal absorbance at 260–280 nm makes it compatible with spectrophotometric assays involving nucleic acids or proteins.
Conclusion
PIPES dipotassium salt (CAS 108321-27-3) is a dependable and versatile biological buffer ideal for near-neutral pH control in biochemical, molecular biology, electrophoretic, and pharmaceutical applications. Its strong buffering capacity, solubility, and compatibility with sensitive biological systems make it a critical tool for accurate and reproducible experimental results.
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