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Product Line: Life Science

3-[N,N-Bis(2-hydroxyethyl)amino]-2-hydroxy-1-propanesulfonic acid

CAS 68399-80-4

Molecular structure of 3-[N,N-Bis(2-hydroxyethyl)amino]-2-hydroxy-1-propanesulfonic acid

CAS · 68399-80-4

01

Product Overview

3-[N,N-Bis(2-hydroxyethyl)amino]-2-hydroxy-1-propanesulfonic acid is a high-purity biological-buffer supplied for industrial and specialty chemical applications. Contact our team for specifications, packaging options and lead times.

02

Product Specifications

Appearance White crystalline powder
Assay 99%~101%
Water 0.5% max
Loss on drying 1% max
Melting point 189~192℃
Solubility (1.0M aqueous) Colorless, clear
pH (1.0M aqueous) 3.0-5.0
Heavy metal (Pb) 0.0005% max
Ultraviolet absorbance/260nm 0.05 max
Ultraviolet absorbance/280nm 0.03 max
03

Applications

1. Biological and Biochemical Buffering Agent

TES is a Good's buffer, widely used in biological and biochemical research to maintain stable pH environments near physiological conditions.

Commonly used in:

Cell culture media

Enzyme assays and protein purification

Molecular biology protocols requiring precise pH control

2. Electrophoresis

Utilized as a buffering component in polyacrylamide gel electrophoresis (PAGE) and other electrophoretic techniques to enhance resolution and reproducibility.

3. Pharmaceutical and Diagnostic Applications

Employed in formulation buffers for pharmaceutical products and diagnostic reagents, providing consistent pH stability during production and storage.

4. Cell Biology and Tissue Engineering

Supports cell viability and growth by buffering culture media without toxicity or interference with biological processes.

04

Benefits

1. Effective pH Buffering in Physiological Range

TES buffers efficiently around neutral pH, ideal for maintaining environments compatible with most biological systems and enzymes.

2. Low Metal Ion Binding

Exhibits minimal binding to divalent cations like Mg²⁺ and Ca²⁺, reducing interference with metal-dependent enzymes or cellular processes.

3. Chemical Stability

Maintains buffering capacity over a range of temperatures and storage conditions without significant degradation.

4. Low UV Absorbance

Transparent in UV and visible spectrum, making it suitable for spectrophotometric and fluorescence assays.

5. Biocompatibility

Non-toxic and non-interfering with cellular metabolism, making it excellent for in vitro and in vivo applications.

05

Conclusion

3-[N,N-Bis(2-hydroxyethyl)amino]-2-hydroxy-1-propanesulfonic acid (TES, CAS 68399-80-4) is a versatile biological buffer that provides stable pH control near physiological levels. Its excellent solubility, low metal binding, and biocompatibility make it a preferred choice for biochemical assays, cell culture, electrophoresis, and pharmaceutical formulations, supporting reliable and reproducible results in life sciences and medical research.

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