CAS · 951-78-0
Product Overview
2-Deoxyuridine is a high-purity pharmaceutical-intermediates supplied for industrial and specialty chemical applications. Contact our team for specifications, packaging options and lead times.
Product Specifications
| Appearance: | White or off-white powder |
|---|---|
| Purity (HPLC): | 99.0% min |
| Solubility (50mg/mL): | Transparent clarification |
| Water: | 1.0% max |
| Specific Optical Rotation: | +50° to +54° (C=1, 1mol/L NaOH) |
| Heavy Metals: | 20 ppm max |
Applications
1. Biochemical and Molecular Biology Research
a. DNA Metabolism Studies
● 2-Deoxyuridine丨CAS 951-78-0 is used as a model compound to study DNA replication, repair, and methylation.
● It is incorporated into DNA in place of thymidine, allowing researchers to investigate mechanisms of base excision repair and uracil-DNA glycosylase activity.
b. Cell Cycle and Apoptosis Research
● Frequently used to synchronize cells in the S phase of the cell cycle.
● Helps in exploring DNA synthesis dynamics and cellular responses to DNA damage.
2. Antiviral and Anticancer Drug Development
a. Precursor for Analog Synthesis
● Serves as a starting material or intermediate in the synthesis of:
Antiviral agents (e.g., idoxuridine, a halogenated derivative)
Antitumor compounds
● These derivatives often show activity by inhibiting DNA synthesis in rapidly dividing cells or viruses.
b. Structural Analog Studies
● Used to study the structure-activity relationships (SARs) of nucleoside analogs for improved pharmacological profiles.
3. Radiolabeling and Tracing
● 2-Deoxyuridine can be radiolabeled (e.g., with tritium or carbon-14) for use in:
DNA synthesis tracking
Nucleoside uptake studies
Cell proliferation assays
4. Gene Expression and Epigenetics
● Utilized in epigenetic research to study cytosine methylation and uracil incorporation as a marker of DNA demethylation processes.
● Assists in understanding genomic instability, particularly in cancer and aging-related research.
5. Enzyme Activity Assays
● Used as a substrate in biochemical assays to assess the activity of enzymes like:
Uracil-DNA glycosylase (UDG)
Thymidine phosphorylase
Deoxyuridine triphosphatase (dUTPase)
These enzymes are significant targets in both diagnostic and therapeutic contexts.
Benefits
1. Versatile Research Tool
● Supports a broad range of studies from basic molecular biology to applied biomedical research.
2. Facilitates Targeted Drug Development
● Enables synthesis and testing of nucleoside analogs with specific antiviral or anticancer properties.
3. Precise DNA Tracking
● When labeled, offers a quantitative method to monitor DNA synthesis, repair, and cell division.
4. Improves Cell Synchronization
● Useful for S-phase synchronization, improving reproducibility in cell-based experiments.
5. Insight into Genomic Stability
● Helps elucidate DNA damage pathways and the role of uracil in mutagenesis and epigenetic regulation.
Conclusion
2-Deoxyuridine丨CAS 951-78-0 is a crucial nucleoside analog widely used in biological research, particularly in studies of DNA synthesis, repair, and epigenetics. As a precursor for important therapeutic compounds and a tool for cellular and molecular investigations, it plays a key role in life sciences, drug development, and cancer research. Its ability to mimic natural DNA components while enabling specific experimental interventions makes it invaluable across multiple scientific disciplines.
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Conclusion
A concise summary and suitability assessment for this product is available on request. Contact our team to discuss whether it fits your process and quality requirements.