CAS · 2650-17-1
Product Overview
Xylene Cyanole FF is a high-purity dyes-pigments supplied for industrial and specialty chemical applications. Contact our team for specifications, packaging options and lead times.
Product Specifications
| Appearance | Green powder |
|---|---|
| Assay | 75% min |
| Identification (IR) | Conforms to structure |
| Solubility | Clear, dark blue solution |
| Loss on drying at 105°C | 10% max |
| Spectrophotometric Assay (Conc. 5.0 mg/L DI Water) | 0.604 min |
| Extinction Co-efficient | 65000 min |
| Suitability | Suitable as a tracking dye for DNA |
Applications
Xylene Cyanole FF is a synthetic tracking dye widely used in molecular biology, biochemistry, and analytical chemistry. It is commonly incorporated into nucleic acid and protein samples to monitor the progress of electrophoretic separation in agarose and polyacrylamide gels. The dye migrates through gels at a predictable rate relative to nucleic acid or protein size, allowing researchers to visually track the movement of samples during gel electrophoresis without affecting the target molecules. Xylene Cyanole FF is also used in DNA and RNA size markers and loading buffers, enabling accurate sample loading and estimation of fragment sizes. Beyond gel electrophoresis, it is employed as a colorimetric indicator in various laboratory protocols where visual monitoring of solution movement or migration is necessary, such as column chromatography or nucleic acid precipitation assays.
Benefits
Xylene Cyanole FF offers several advantages that make it an essential tool in laboratory workflows. Its predictable migration behavior provides reliable tracking of nucleic acid and protein samples, helping prevent over-running or incomplete separation during electrophoresis. The dye does not bind strongly to nucleic acids or proteins, minimizing interference with downstream analysis such as staining, blotting, or enzymatic reactions. Its intense color ensures easy visualization of sample migration even at low concentrations, enhancing accuracy during gel loading and monitoring. Xylene Cyanole FF is highly soluble in common electrophoresis buffers and remains stable under standard experimental conditions, providing consistent performance across multiple experiments. Additionally, it allows for simultaneous monitoring of multiple sample types when combined with other dyes, improving the efficiency of gel-based analyses.
Conclusion
Xylene Cyanole FF is a reliable and widely used tracking dye in molecular biology and analytical laboratories. Its predictable migration, minimal interference with target molecules, and strong visible coloration make it ideal for monitoring nucleic acid and protein electrophoresis, as well as other laboratory processes requiring visual tracking. By enhancing experimental control, accuracy, and reproducibility, Xylene Cyanole FF supports efficient and effective laboratory workflows across a variety of molecular and biochemical applications.
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