CAS · 6976-37-0
Product Overview
Bis-Tris丨CAS 6976-37-0 is a widely used biological buffer compound, notable for its ability to maintain stable pH conditions in biochemical and molecular biology experiments. It is part of the tris-based buffer family, designed to provide buffering capacity in the pH range of approximately 5.8 to 7.2, making it particularly useful for neutral to slightly acidic conditions.
Product Specifications
| Appearance | White crystalline powder |
|---|---|
| Assay | 98% min |
| Water Content | 1.0% max |
| pH (1% aq.) | 9.5 - 11.0 |
| Solubility (1% aq.) | Clear, colorless solution |
| UV Absorbance @ 280nm (0.1M aq.) | 0.04 max |
| UV Absorbance @ 340nm (0.1M aq.) | 0.02 max |
Applications
1. Biochemical and Molecular Biology Buffering
Used extensively to maintain physiological pH in enzyme reactions, protein purification, and nucleic acid work.
Ideal for buffers requiring stable pH around 6.5, such as in assays involving pH-sensitive enzymes or proteins.
Commonly used in electrophoresis buffers, cell culture media, and chromatography buffers.
2. Protein Purification and Crystallization
Bis-Tris丨CAS 6976-37-0 provides stable buffering conditions during protein purification processes such as ion-exchange chromatography and size-exclusion chromatography.
Used in crystallization buffers to help stabilize protein structure and improve crystal quality.
3. Pharmaceutical and Diagnostic Formulations
Employed in formulations of drugs and vaccines where maintaining specific pH conditions is critical for stability.
Used in immunoassays and other diagnostic kits requiring precise pH control.
4. Enzyme Assays and Biochemical Studies
Supports enzymatic activity by maintaining a consistent pH environment.
Used in the study of enzymes with optimum activity near neutral pH.
Benefits
1. Effective Buffering Range
Buffers effectively in the pH range 5.8 to 7.2, making it well-suited for near-neutral pH applications.
pKa ≈ 6.5 at 25°C, ensuring good buffering capacity in this range.
2. Low Temperature Sensitivity
Unlike some buffers, Bis-Tris exhibits minimal changes in buffering capacity with temperature variations, useful in experiments conducted at varying temperatures.
3. Minimal Metal Ion Binding
Shows less chelation of divalent metal ions (e.g., Mg²⁺, Ca²⁺) compared to other buffers like phosphate, thus minimizing interference in metal-dependent enzymatic reactions.
4. High Water Solubility and Stability
Highly soluble in water and chemically stable over a broad pH and temperature range.
Compatible with many biological molecules and reagents.
5. Compatibility
Compatible with a wide variety of biological molecules, including proteins, nucleic acids, and membranes.
Does not absorb significantly in UV range, facilitating spectrophotometric assays.
Conclusion
A concise summary and suitability assessment for this product is available on request. Contact our team to discuss whether it fits your process and quality requirements.