CAS · 1132-61-2
Product Overview
3-Morpholinopropanesulfonic acid is a high-purity biological-buffer supplied for industrial and specialty chemical applications. Contact our team for specifications, packaging options and lead times.
Product Specifications
| Appearance | White crystalline powder |
|---|---|
| Assay | 99%~101% |
| Water | 0.5% max |
| Loss on drying | 1% max |
| Melt point | 279~281℃ |
| Solubility (1.0M aqueous) | Colorless, transparent |
| pH (1.0M aqueous) | 3.6~4.4 |
| Cl | 0.05% max |
| SO4 | 0.005% max |
| Pb | 0.0005% max |
| Ultraviolet absorbance (260nm) | 0.05% max |
| Ultraviolet absorbance (280nm) | 0.03% max |
Applications
1. Biological and Biochemical Research
MOPS is a Good's buffer, designed specifically for biological systems.
Commonly used in:
Protein purification and enzymatic assays
Electrophoresis (especially RNA gel electrophoresis)
Cell-free expression systems
Often preferred for studies requiring minimal interaction with metal ions or biological macromolecules.
2. Cell Culture and Microbiology
Used in bacterial growth media (e.g., MOPS minimal media) for maintaining a stable pH during cell growth and fermentation.
Helps optimize microbial metabolic pathways in bioprocessing research and industrial microbiology.
3. Diagnostic and Analytical Reagents
Employed in clinical chemistry and diagnostic kits, including PCR and nucleic acid assays, where consistent pH is critical to reaction performance.
4. Pharmaceutical Formulations
Sometimes used in buffer formulations for injectable or oral drug preparations, where stability near physiological pH is required.
Benefits
1. Excellent Buffering Near Physiological pH
Effective pKa of ~7.2 makes MOPS ideal for maintaining neutral to slightly basic pH, suitable for most cellular and enzymatic systems.
2. Low Metal Ion Binding
Unlike phosphate or EDTA buffers, MOPS exhibits minimal interaction with divalent cations (e.g., Mg²⁺, Ca²⁺), making it compatible with metal-dependent enzymes and cofactors.
3. High Solubility and Stability
Forms clear, aqueous solutions and maintains buffering capacity over a wide range of temperatures and concentrations.
Non-volatile and chemically stable, even under extended storage.
4. Low UV Absorbance
Minimal absorbance at 260 nm and 280 nm, making it ideal for spectrophotometric assays involving nucleic acids and proteins.
5. Biocompatible and Non-toxic
Designed to be non-interfering in biological systems, MOPS is suitable for long-term biological experiments and live cell studies.
Conclusion
3-Morpholinopropanesulfonic acid (MOPS, CAS 1132-61-2) is a highly regarded biological buffer used extensively in life sciences, microbiology, and diagnostics. Its neutral pKa, low metal binding, and UV transparency make it a preferred choice for experiments involving RNA, enzymes, or live cells. Whether in high-precision lab work or scalable industrial bioprocesses, MOPS offers stable and reliable pH control essential for reproducible results.
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