CAS · 105931-58-6
Product Overview
N6-Methyl-dAphosphoramidite is a high-purity nucleosides-nucleotides supplied for industrial and specialty chemical applications. Contact our team for specifications, packaging options and lead times.
Product Specifications
| Appearance: | White to light yellow amorphous powder |
|---|---|
| Purity (HPLC): | 98% min |
| Solubility Clarity: | 0.2M solution in acetonitrile is free from undissolved particulate |
| Identity (by 1H NMR): | To conform structure |
| 31P NMR: | 98% min |
| Residual Solvent: | 3.0% max |
| Moisture: | 0.5% max |
Applications
N6-Methyl-dAphosphoramidite is a specialized nucleotide derivative widely used in oligonucleotide synthesis and nucleic acid research. It serves as a building block for the preparation of synthetic DNA strands containing site-specific N6-methyladenosine modifications. Such modified oligonucleotides are essential for studying epigenetic regulation, RNA modification pathways, and DNA-protein interactions. In addition, it is employed in the development of antisense oligonucleotides, aptamers, and other nucleic acid-based therapeutics, where precise incorporation of methylated nucleotides can influence binding affinity, stability, and biological activity. The compound is also valuable in chemical biology for probing methylation-dependent processes, enzyme specificity, and epigenetic marker detection.
Benefits
The compound offers significant advantages due to its high specificity, reactivity, and stability. The phosphoramidite group allows for efficient and controlled coupling during automated DNA synthesis, ensuring precise site-specific incorporation of N6-methyladenosine. This enables researchers to generate oligonucleotides with defined epigenetic modifications for functional studies. The N6-methyl modification enhances resistance to enzymatic degradation and can modulate base-pairing properties, which is valuable in both therapeutic design and molecular biology research. Additionally, the compound is compatible with standard solid-phase oligonucleotide synthesis protocols, making it convenient for laboratory and industrial applications.
Conclusion
N6-Methyl-dAphosphoramidite is a critical building block for epigenetics research, nucleic acid therapeutics, and chemical biology. Its ability to enable site-specific incorporation of methylated adenosine into DNA or RNA strands provides researchers and developers with precise control over oligonucleotide structure and function. With applications in synthetic biology, drug development, and molecular studies, it is indispensable for advancing the understanding and utilization of nucleotide modifications in both research and applied contexts.
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